Improving Salt Stress tolerance in Tomato ny Engineering a Ubiquitination Resistant Transcription Factor
Salt stress is one of the major factors causing severe crop loss in the US. Many genes have been demonstrated as playing a significant role in salt stress tolerance by rapid induction in response to salt stress. We have identified a tomato (Solanum lycopersicum) gene encoding a NAC transcription factor (SlNAC1) that is strongly induced in response to salt stress. Significantly, the SlNAC1 protein is degraded via a ubiquitination-mediated proteolysis. Together these data indicate the regulation of this transcription factor is fine-tuned at both transcriptional and post-translational levels. We will study the function and regulation of SlNAC1 under the salt stress condition. Additionally, unlike using the conventional approach of simple over-expression of a gene involved in salt stress response, we propose a novel strategy to improve the tolerance
of salt stress in tomato by generating transgenic plants conditionally expressing an engineered degradation-resistant transcription factor.