Dual reporter system for in situ detection of plasmid transfer under aerobic and anaerobic conditions. Academic Article uri icon

Overview

abstract

  • We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.

publication date

  • January 1, 2010

Research

keywords

  • Aerobiosis
  • Anaerobiosis
  • Bacteriophage T7
  • Conjugation, Genetic
  • DNA Transposable Elements
  • DNA-Directed RNA Polymerases
  • Escherichia coli
  • Genetic Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Plasmids
  • Promoter Regions, Genetic
  • Viral Proteins

Identity

PubMed ID

  • 20453134

Additional Document Info

start page

  • 4553

end page

  • 4556

volume

  • 76

issue

  • 13