Publications in VIVO

Co-author Network

Co-investigator Network

Carolyn Hovde Bohach University Distinguished Professor and Director, Idaho INBRE

Positions

Director, Idaho INBRE
University Distinguished Professor, Animal, Veterinary & Food Sciences , College of Agricultural & Life Sciences

Hovde Bohach’s area of expertise is infectious disease with emphasis on the foodborne pathogen E. coli O157:H7. Primary interests are in the relationship of this human pathogen with healthy cattle.

Research Areas

Publications

selected publications

academic article
- Building a biomedical pipeline: the impact of the Idaho IDeA INBRE summer research experience at a primarily undergraduate institution. Advances in Physiology Education. 45:786-796. 2021
- Deletion of Yersinia pestis ail Causes Temperature-Sensitive Pleiotropic Effects, Including Cell Lysis, That Are Suppressed by Carbon Source, Cations, or Loss of Phospholipase A Activity. Journal of Bacteriology. 203.0. 2021
- Repeated Oral Vaccination of Cattle with Shiga toxin-negative Escherichia coli O157:H7 Reduces Carriage of Wild-type E. coli O157:H7 after Challenge. Applied and Environmental Microbiology. 87:e02183-20. 2021
- The interactions of bacteriophage Ace and Shiga toxin-producing Escherichia coli during biocontrol. FEMS Microbiology Ecology. 97. 2021
- Escherichia coli O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle. Microorganisms. 8:580. 2020
- Author's Perspectives on Their Highly Cited Papers Published in Foodborne Pathogens and Disease. Foodborne Pathogens And Disease. 15:03-10. 2018
- Complete Genome Sequence of Broad-Host-Range Shiga Toxin-Converting Bacteriophage SH2026Stx1, Isolated from Escherichia coli O157:H7. Genome Announcements. 6:UNSP e00490-18. 2018
- High-Quality Complete Genome Sequences of Three Bovine Shiga Toxin-Producing Escherichia coli O177:H- (fliC(H25)) Isolates Harboring Virulent stx2 and Multiple Plasmids. Microbiology Resource Announcements. 6:UNSP e01592-17. 2018
- Rapid Evolution of Citrate Utilization by Escherichia coli by Direct Selection Requires citT and dctA. Journal of Bacteriology. 198:1022-1034. 2016
- Standardized Escherichia coli O157:H7 Exposure Studies in Cattle Provide Evidence that Bovine Factors Do Not Drive Increased Summertime Colonization. Applied and Environmental Microbiology. 82:964-971. 2016
- Impact of Infection Dose and Previous Serum Antibodies against the Locus of Enterocyte Effacement Proteins on Escherichia coli O157:H7 Shedding in Calves following Experimental Infection. Biomed Research International. 2015
- The Escherichia coli O157:H7 cattle immunoproteome includes outer membrane protein A (OmpA), a modulator of adherence to bovine rectoanal junction squamous epithelial (RSE) cells. Proteomics. 15:1829-1842. 2015
- Physiological Levels of Glucose Induce Membrane Vesicle Secretion and Affect the Lipid and Protein Composition of Yersinia pestis Cell Surfaces. Applied and Environmental Microbiology. 79:4509-4514. 2013
- SdiA aids enterohemorrhagic Escherichia coli carriage by cattle fed a forage or grain diet. Infection and Immunity. 81:3472-3478. 2013
- The Acyl-Homoserine Lactone Synthase Yenl from Yersinia enterocolitica Modulates Virulence Gene Expression in Enterohemorrhagic Escherichia coli. Infection and Immunity. 81:4192-4199. 2013
- Differential Virulence of Clinical and Bovine-Biased Enterohemorrhagic Escherichia coli O157:H7 Genotypes in Piglet and Dutch Belted Rabbit Models. Infection and Immunity. 80:369-380. 2012
- Role of a New Intimin/Invasin-Like Protein in Yersinia pestis Virulence. Infection and Immunity. 80:3559-3569. 2012
- Yersinia pestis Ail: multiple roles of a single protein. Frontiers in Cellular and Infection Microbiology. 103. 2012
- Internalization of Escherichia coli o157:h7 by bovine rectal epithelial cells. Frontiers in Microbiology. 2:32. 2011
- Escherichia coli O157:H7: Animal Reservoir and Sources of Human Infection. Foodborne Pathogens And Disease. 8:465-487. 2011
- Chemical sensing in mammalian host-bacterial commensal associations. Proceedings of the National Academy of Sciences of the United States of America. 107:9831-9836. 2010
- Effect of Saccharomyces cerevisiae fermentation product on ruminal fermentation and nutrient utilization in dairy cows. Journal of Dairy Science. 93:682-692. 2010
- A Brief Overview of Escherichia coli O157:H7 and Its Plasmid O157. Journal of Microbiology and Biotechnology. 20:5-14. 2010
- Characterization of BNT2, an intrinsically curved DNA of Escherichia coli O157:H7. Biochemical and Biophysical Research Communications. 391:1792-1797. 2010
- Influence of plasmid pO157 on Escherichia coli O157:H7 Sakai biofilm formation.. Applied and Environmental Microbiology. 76. 2010
- Outer Membrane Protein X (Ail) Contributes to Yersinia pestis Virulence in Pneumonic Plague and Its Activity Is Dependent on the Lipopolysaccharide Core Length. Infection and Immunity. 78:5233-5243. 2010
- Phenotypic diversity of Escherichia coli O157:H7 strains associated with the plasmid O157. Journal of Microbiology. 48:347-357. 2010
- All blood, No stool: enterohemorrhagic Escherichia coli O157 : H7 infection. Journal of Veterinary Science. 9:219-231. 2008
- Characterization of an Escherichia coli O157:H7 O-antigen deletion mutant and effect of the deletion on bacterial persistence in the mouse intestine and colonization at the bovine terminal rectal mucosa.. Applied and Environmental Microbiology. 74. 2008
- Induction of innate immunity by lipid A mimetics increases survival from pneumonic plague. Microbiology. 154:2131-2138. 2008
- Lipid A mimetics are potent adjuvants for an intranasal pneumonic plague vaccine. Vaccine. 26:5554-5561. 2008
- Low numbers of intestinal Shiga toxin-producing E. coli correlate with a poor prognosis in sheep infected with bovine leukemia virus. Journal of Veterinary Science. 9:375-379. 2008
- Nanowire-based delivery of Escherichia coli O157 shiga toxin 1 A subunit into human and bovine cells. Nano Letters. 7. 2007
- Characterization of an Escherichia coli O157:H7 plasmid O157 deletion mutant and its survival and persistence in cattle.. Applied and Environmental Microbiology. 73. 2007
- Escherichia coli O157:H7 colonization at the rectoanal junction of long-duration culture-positive cattle. Applied and Environmental Microbiology. 73. 2007
- Expression profiles of bovine genes in the rectoanal junction mucosa during colonization with Escherichia coli O157:H7. Applied and Environmental Microbiology. 73. 2007
- Phenotypic characterization of OmpX, an Ail homologue of Yersinia pestis KIM. Microbiology. 153:2941-2951. 2007
- Rectoanal junction colonization of feedlot cattle by Escherichia coli O157 : H7 and its association with supershedders and excretion dynamics. Applied and Environmental Microbiology. 73:1563-1568. 2007
- The non-toxic A subunit of Shiga toxin type 1 prevents replication of bovine immunodeficiency virus in infected cells. Virus Research. 125:29-41. 2007
- Comparison of a waterless hand-hygiene preparation and soap-and-water hand washing to reduce coliforms on hands in animal exhibit settings. Epidemiology and Infection. 134:1024-1028. 2006
- Application of bacteriophages to control intestinal Escherichia coli O157: H7 levels in ruminants. Applied and Environmental Microbiology. 72:5359-5366. 2006
- Comparison of cultures from rectoanal-junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers.. Applied and Environmental Microbiology. 72. 2006
- Intestinal Shiga toxin-producing Escherichia coli bacteria mitigate bovine leukemia virus infection in experimentally infected sheep.. Infection and Immunity. 74. 2006
- Proteomics-based expression library screening (PELS) - A novel method for rapidly defining microbial immunoproteomes. Molecular and Cellular Proteomics. 5:1514-1519. 2006
- Role of Escherichia coli O157:H7 virulence factors in colonization at the bovine terminal rectal mucosa. Infection and Immunity. 74. 2006
- The Escherichia coli O157 flagellar regulatory gene flhC and not the flagellin gene fliC impacts colonization of cattle. Infection and Immunity. 74. 2006
- Characterization of a Shiga toxin-, intimin-, and enterotoxin hemolysin-producing Escherichia coli ONT : H25 strain commonly isolated from healthy cattle. Journal of Clinical Microbiology. 43:3213-3220. 2005
- Escherichia coli O157:H7 in environments of culture-positive cattle.. Applied and Environmental Microbiology. 71. 2005
- Involvement of the Escherichia coli O157:H7(pO157) ecf operon and lipid A myristoyl transferase activity in bacterial survival in the bovine gastrointestinal tract and bacterial persistence in farm water troughs.. Infection and Immunity. 73. 2005
- Heat inactivation of E-coli during manure composting. Compost Science and Utilization. 12:314-322. 2004
- Rectal administration of Escherichia coli O157:H7: novel model for colonization of ruminants.. Applied and Environmental Microbiology. 70. 2004
- Shiga toxin 1 targets bovine leukemia virus-expressing cells.. Infection and Immunity. 72. 2004
- Thermoregulation of the Escherichia coli O157:H7 pO157 ecf operon and lipid A myristoyl transferase activity involves intrinsically curved DNA.. Molecular Microbiology. 51. 2004
- Correlation between geographic distance and genetic similarity in an international collection of bovine faecal Escherichia coli O157 : H7 isolates. Epidemiology and Infection. 131:923-930. 2003
- Acid resistance of Escherichia coli O157:H7 from the gastrointestinal tract of cattle fed hay or grain. Veterinary Microbiology. 95. 2003
- Antiviral activity of shiga toxin requires enzymatic activity and is associated with increased permeability of the target cells.. Infection and Immunity. 71. 2003
- Rectoanal mucosal swab culture is more sensitive than fecal culture and distinguishes Escherichia coli O157 : H7-colonized cattle and those transiently shedding the same organism. Journal of Clinical Microbiology. 41:4924-4929. 2003
- Gastrointestinal tract location of Escherichia coli O157:H7 in ruminants.. Applied and Environmental Microbiology. 68. 2002
- Ruminant gastrointestinal cell proliferation and clearance of Escherichia coli O157:H7. Infection and Immunity. 68. 2000
- Antiviral activity of shiga toxin 1: suppression of bovine leukemia virus-related spontaneous lymphocyte proliferation.. Infection and Immunity. 68. 2000
- Cattle, hay, and E-coli. Science (New York, N.Y). 284:51-52. 1999
- Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Applied and Environmental Microbiology. 65. 1999
- Effect of cattle diet on Escherichia coli O157:H7 acid resistance.. Applied and Environmental Microbiology. 65. 1999
- Shiga toxin attacks bacterial ribosomes as effectively as eucaryotic ribosomes. Biochemistry. 37:9394-9398. 1998
- Analysis of Escherichia coli O157:H7 survival in ovine or bovine manure and manure slurry. Applied and Environmental Microbiology. 64. 1998
- Disruption of an internal membrane-spanning region in Shiga toxin 1 reduces cytotoxicity. Infection and Immunity. 66. 1998
- Evaluation of Dietary Influences on Escherichia coli O157:H7 Shedding by Sheep. Applied and Environmental Microbiology. 63. 1997
- Purification of Staphylococcus aureus beta-toxin: Comparison of three isoelectric focusing methods. Protein Expression and Purification. 9:76-82. 1997
- Characterization of Escherichia coli O157:H7 and other Shiga toxin-producing E-coli serotypes isolated from sheep. Journal of Clinical Microbiology. 35:892-899. 1997
- A minimal medium for growth of Pasteurella multocida. FEMS Microbiology Letters. 140:165-169. 1996
- Escherichia coli O157:H7 in microbial flora of sheep. Journal of Clinical Microbiology. 34:431-433. 1996
- Identification of Pasteurella multocida tryptophan synthase beta-subunit by antisera against strain p1059. Microbiology. 142:115-121. 1996
- Effect of diet on the shedding of Escherichia coli O157:H7 in a sheep model.. Applied and Environmental Microbiology. 61. 1995
- Purification of recombinant Shiga-like toxin type I B subunit. Protein Expression and Purification. 6:771-779. 1995
- Investigation of the role of the disulphide bond in the activity and structure of staphylococcal enterotoxin C1. Molecular Microbiology. 13:897-909. 1994
- Purification of recombinant Shiga-like toxin type-1 A(1) fragment from Escherichia-coli. Protein Expression and Purification. 5:291-295. 1994
- Evidence that the A2 fragment of Shiga-like toxin type I is required for holotoxin integrity.. Infection and Immunity. 62. 1994
- The role of tyrosine-114 in the enzymatic-activity of the Shiga-like toxin-1 A-chain. Molecular and General Genetics. 241:467-473. 1993
- Nucleotide-sequence of the Staphylococcal enterotoxin-C3 gene sequence comparison of all 3 type-C Staphylococcal enterotoxins. Molecular and General Genetics. 220:329-333. 1990
conference paper
- Local and systemic lymphoproliferative responses to Escherichia coli O157:H7 proteins occur after colonization and are predictive of decreased fecal shedding.. Journal of Immunology. 2012
- The effect of dietary protein, fiber, and digesta passage rate on the duration and concentration of Escherichia coli O157: H7 shed by cattle. American Association of Bovine Practitioners Conference. 1998

featured in

Carolyn Bohach Named an AAAS Fellow News Release

Research

principal investigator on

Idaho INBRE Program (FY 2018) awarded by National Institute of General Medical Sciences (NIGMS) 2018 - 2019
Administrative Core (FY 2019) awarded by National Institute of General Medical Sciences (NIGMS) 2018 - 2019
Factors That Influence Cattle Colonization with E. Coli O157:h7 awarded by National Institute for Food and Agriculture 2012 - 2017
Idaho INBRE Program (FY2013) awarded by National Institute of General Medical Sciences (NIGMS) 2013 - 2014
Idaho INBRE Program (FY2012) awarded by National Institute of General Medical Sciences (NIGMS) 2012 - 2013
Genetic Characterization of E. Coli O157:h7 Colonization of Cattle awarded by National Institute for Food and Agriculture 2007 - 2012
Administrative Core (FY 2011) awarded by National Center for Research Resources (NCRR) 2011 - 2012
Bioinformatics Core (FY 2011) awarded by National Center for Research Resources (NCRR) 2011 - 2012
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2011 - 2012
Outreach Core: Enhancements Of Traditional Teaching Colleges awarded by National Center for Research Resources (NCRR) 2011 - 2012
Outreach Core: Pipeline To Graduate Education awarded by National Center for Research Resources (NCRR) 2011 - 2012
Research Core: Core Research Facilities & Infrastructure awarded by National Center for Research Resources (NCRR) 2011 - 2012
Research Core: Faculty Development awarded by National Center for Research Resources (NCRR) 2011 - 2012
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2009 - 2012
Administrative Core (FY 2010) awarded by National Center for Research Resources (NCRR) 2010 - 2011
Bioinformatics Core (FY 2010) awarded by National Center for Research Resources (NCRR) 2010 - 2011
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2010 - 2011
Outreach Core: Enhancements Of Traditional Teaching Colleges awarded by National Center for Research Resources (NCRR) 2010 - 2011
Outreach Core: Pipeline To Graduate Education awarded by National Center for Research Resources (NCRR) 2010 - 2011
Research Core: Core Research Facilities & Infrastructure awarded by National Center for Research Resources (NCRR) 2010 - 2011
Research Core: Faculty Development awarded by National Center for Research Resources (NCRR) 2010 - 2011
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2009 - 2011
Administrative Core (FY 2009) awarded by National Center for Research Resources (NCRR) 2009 - 2010
Bioinformatics Core (FY 2009) awarded by National Center for Research Resources (NCRR) 2009 - 2010
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2009 - 2010
Outreach Core: Enhancements Of Traditional Teaching Colleges awarded by National Center for Research Resources (NCRR) 2009 - 2010
Outreach Core: Pipeline To Graduate Education awarded by National Center for Research Resources (NCRR) 2009 - 2010
Research Core: Core Research Facilities & Infrastructure awarded by National Center for Research Resources (NCRR) 2009 - 2010
Research Core: Faculty Development Program awarded by National Center for Research Resources (NCRR) 2009 - 2010
The Role Of Novel Surface Proteins In Yersinia Pestis Virulence awarded by National Center for Research Resources (NCRR) 2008 - 2009
Administrative Core (FY 2008) awarded by National Center for Research Resources (NCRR) 2008 - 2009
Bioinformatics Core (FY 2008) awarded by National Center for Research Resources (NCRR) 2008 - 2009
Outreach Core: Enhancements Of Traditional Teaching Colleges awarded by National Center for Research Resources (NCRR) 2008 - 2009
Outreach Core: Pipeline To Graduate Education awarded by National Center for Research Resources (NCRR) 2008 - 2009
Research Core: Core Research Facilities & Infrastructure awarded by National Center for Research Resources (NCRR) 2008 - 2009
Research Core: Faculty Development Program awarded by National Center for Research Resources (NCRR) 2008 - 2009
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2008 - 2009
E. Coli O15:h7 and Cattle: Genetic Characterization of the Host-Bacteria Interaction awarded by National Institute for Food and Agriculture 2004 - 2008
The Role Of Novel Surface Proteins In Yersinia Pestis Virulence awarded by National Center for Research Resources (NCRR) 2007 - 2008
Administrative Core (FY 2007) awarded by National Center for Research Resources (NCRR) 2007 - 2008
Bioinformatics Core (FY 2007) awarded by National Center for Research Resources (NCRR) 2007 - 2008
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2007 - 2008
Outreach Core: Enhancements Of Traditional Teaching Colleges awarded by National Center for Research Resources (NCRR) 2007 - 2008
Outreach Core: Pipeline To Graduate Education awarded by National Center for Research Resources (NCRR) 2007 - 2008
Research Core: Core Research Facilities & Infrastructure awarded by National Center for Research Resources (NCRR) 2007 - 2008
Research Core: Faculty Development Program awarded by National Center for Research Resources (NCRR) 2007 - 2008
Antiviral Activity of Shiga Toxin Towards Bovine Leukemia Virus awarded by National Institute for Food and Agriculture 2002 - 2007
Idaho INBRE Program awarded by National Center for Research Resources (NCRR) 2006 - 2007
COBRE: UID: Antiviral Activity Of E Coli Shiga Toxin (FY 2005) awarded by National Center for Research Resources (NCRR) 2005 - 2006
COBRE: UID: Antiviral Activity Of E Coli Shiga Toxin (FY 2004) awarded by National Center for Research Resources (NCRR) 2004 - 2005
Identifying Factors that Promote Clearance of E. Coli O157:h7 from Cattle awarded by National Institute for Food and Agriculture 1999 - 2003
Escherichia Coli Elimination In Cattle awarded by National Institute of Child Health and Human Development (NICHD) 2000 - 2002
Escherichia Coli Elimination In Cattle awarded by National Institute of Child Health and Human Development (NICHD) 2000 - 2001
Structure-Function Analysis Of Shiga-Like Toxin awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1997 - 1999
Structure-Function Analysis Of Shiga-Like Toxin awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1996 - 1997
Structure-Function Analysis Of Shiga-Like Toxin awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1995 - 1996
Small Instrumentation Grant awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1994 - 1996
Structure-Function Analysis Of Shiga-Like Toxin awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1994 - 1995
Structure-Function Analysis Of Shiga-Like Toxin awarded by National Institute of Allergy and Infectious Diseases (NIAID) 1993 - 1994

co-principal investigator on

Colonization Characteristics of Bovine Recto-anal Junction Tissue by Escherichia Coli O157:h7 awarded by USDA Agricultural Research Service 2007 - 2008
Bovine Functional Genomics in Lactation, Food Safety, and Mastitis awarded by National Institute for Food and Agriculture 2004 - 2005

Background

education and training

Ph.D., University of Minnesota 1985
M.S., Swedish Hospital Medical Center , MT (ASCP) 1976
B.S., University of Illinois 1975

awards and honors

Outstanding Faculty Award, conferred by UI Student Disability Services
R.M. Wade Excellence in Teaching Award, conferred by College of Agricultural & Life Sciences
Award for Faculty Excellence, conferred by University of Idaho Alumni Association
Al Thompson Award, conferred by Northwest Association for Biomedical Research
Faculty Member of the Year, conferred by University of Idaho
Excellence in Outreach and Engagement Award, conferred by University of Idaho
Honorary Professor, conferred by Jiangsu Academy of Agricultural Sciences (JAAS)
Distinguished Undergraduate Teaching Award, conferred by Carski Foundation

Contact

full name

Dr. Carolyn Hovde Bohach