Pyrene-functionalized oligonucleotides and locked nucleic acids (LNAs): tools for fundamental research, diagnostics, and nanotechnology Academic Article Review uri icon

Overview

abstract

  • Pyrene-functionalized oligonucleotides (PFOs) are increasingly explored as tools in fundamental research, diagnostics and nanotechnology. Their popularity is linked to the ability of pyrenes to function as polarity-sensitive and quenchable fluorophores, excimer-generating units, aromatic stacking moieties and nucleic acid duplex intercalators. These characteristics have enabled development of PFOs for detection of complementary DNA/RNA targets, discrimination of single nucleotide polymorphisms (SNPs), and generation of ?-arrays on nucleic acid scaffolds. This critical review will highlight the physical properties and applications of PFOs that are likely to provide high degree of positional control of the chromophore in nucleic acid complexes. Particular emphasis will be placed on pyrene-functionalized Locked Nucleic Acids (LNAs) since these materials display interesting properties such as fluorescence quantum yields approaching unity and recognition of mixed-sequence double stranded DNA (144 references).

publication date

  • 2011

Research

keywords

  • 3T3-L1 Cells
  • Animals
  • Base Sequence
  • DNA
  • Humans
  • Mice
  • Nanotechnology
  • Oligonucleotides
  • Pyrenes

Identity

PubMed ID

  • 21487621

Additional Document Info

start page

  • 5771

end page

  • 5788

volume

  • 40

number

  • 12