Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae. Academic Article uri icon

Overview

abstract

  • Autophagy is essential for prolonging yeast survival during nutrient deprivation; however, this report shows that some autophagy proteins may also be accelerating population death in those conditions. While leucine starvation caused YCA1-mediated apoptosis characterized by increased annexin V staining, nitrogen deprivation triggered necrotic death characterized by increased propidium iodide uptake. Although a ?atg8 strain died faster than its parental strain during nitrogen starvation, this mutant died slower than its parent during leucine starvation. Conversely, a ?atg11 strain died slower than its parent during nitrogen starvation, but faster during leucine starvation. Curiously, although GFP-Atg8 complemented the ?atg8 mutation, this protein made ATG8 cells more sensitive to nitrogen starvation, and less sensitive to leucine starvation. These results were difficult to explain if autophagy only extended life but could be an indication that a second form of autophagy could concurrently facilitate either apoptotic or necrotic cell death.

publication date

  • January 4, 2013

Research

keywords

  • Apoptosis
  • Autophagy
  • Cytoprotection
  • Green Fluorescent Proteins
  • Leucine
  • Microbial Viability
  • Mutation
  • Nitrogen
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sirolimus

Identity

PubMed ID

  • 22361650

Additional Document Info

start page

  • 731

end page

  • 738

volume

  • 8

number

  • 5