Application of New Diagnostic Reagents for Identification of Diverse and Newly Identified Strains of Potato Virus Y (FY 2012)
Purified nucleoproteins of newly identified strains of potato virus Y will be purified in the laboratory of the SY with the U. of Idaho. New antibodies will be produced and screened. Promising materials will be sent to the ARS laboratory in Prosser for additional tests with the numerous isolates of potato virus Y maintained by the ARS researcher. Results will be relayed back to Un of Idaho. Similarly, genomic sequence information will be used to design new sets of RT-PCR primers. These will be tested for detection and identification of diverse strains of potato virus Y by both Un of Idaho and ARS.