The Role Of Novel Surface Proteins In Yersinia Pestis Virulence
Yersinia pestis, the etiologic agent of bubonic and pneumonic plague, encodes several unique surface proteins including a large membrane associated protein classed within the invasin-family of proteins. This protein, encoded by a gene ca. 10kb of DNA (gene designation YPO3944) has not been characterized to date and its role in pathogenesis is unknown. Our specific goals for this project are to:1. Determine the regulation of this gene under various environmental conditions using the phoA reporter system and real time PCR.2. Generate a deletion mutation by site-directed mutagenesis and compare the virulence of this mutant with the wild-type parental strain in cell culture invasion assays and the mouse pathogenesis model.