The Role Of Novel Surface Proteins In Yersinia Pestis Virulence Grant uri icon

Overview

abstract

  • Yersinia pestis, the etiologic agent of bubonic and pneumonic plague, encodes several unique surface proteins including a large membrane associated protein classed within the invasin-family of proteins.  This protein, encoded by a gene ca. 10kb of DNA (gene designation YPO3944)  has not been characterized to date and its role in pathogenesis is unknown.  Our specific goals for this project are to:1.  Determine the regulation of this gene under various environmental conditions using the phoA reporter system and real time PCR.2.  Generate a deletion mutation by site-directed mutagenesis and compare the virulence of this mutant with the wild-type parental strain in cell culture invasion assays and the mouse pathogenesis model.

date/time interval

  • June 1, 2007 - May 31, 2008

sponsor award ID

  • 5P20RR015587-08

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